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PRP-X100色譜柱

更新時間:2018-07-06      點擊次數(shù):2320

Hamilton Company專注于精密測量設備,自動化液體處理工作站和樣品管理系統(tǒng)的開發(fā),制造和定制。Hamilton的流程針對質量和靈活性進行了優(yōu)化。無論是具有快速交付時間框架的定制針頭,特殊長度的pH傳感器,還是全面自動化您的檢測工作流程的全面解決方案,相信Hamilton產品將始終滿足您的需求。

Hamilton公司60多年來一直是的制造商,總部位于內華達州里諾市; 馬薩諸塞州富蘭克林; 蒂米什瓦拉,羅馬尼亞; 和瑞士的Bonaduz; 和遍布的子公司。

 

PRP-X100色譜柱

可靠的有機和無機陰離子分離

Hamilton PRP-X100和PRP-X110是高度穩(wěn)定的惰性材料。PRP-X100幾乎可用于任何HPLC或離子色譜儀,包括IC單元。現(xiàn)代聚合物化學技術的進步現(xiàn)在提供了更堅固的色譜柱,其分離效率比以前的前代產品高得多。PRP-X100和PRP-X110色譜柱非常適用于采用抑制/非抑制電導率,電化學,UV和ICP-MS檢測的系統(tǒng)。目前使用濕法化學或比色法的色譜工作者將發(fā)現(xiàn)離子色譜法大大減少了樣品預處理并提高了結果的準確性和度。

 

HPLC樹脂規(guī)格

Reversed Phase Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-1

PSDVB*

N/A

100 Å

5-85 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-C18

C18 bonded to PSDVB*

N/A

100 Å

5-85 °C

pH 1 – 13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-3

PSDVB*

N/A

300 Å

5-85 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-h5

Fluorinated PSDVB*

N/A

300 Å

5-85 °C

pH 1 – 13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

HxSil C18

Silica

N/A

110 Å

5-60 °C

pH 1.5 – 10. 0-100% aqueous, organic modifier

N/A

6,000 psi

HxSil C8

Silica

N/A

110 Å

5-60 °C

pH 1.5 – 10. 0-100% aqueous, organic modifier

N/A

6,000 psi

Anion Exchange Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X100

PSDVB* with Tri- methyl ammonium Exchanger

0.19meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X110

PSDVB* with Tri-methyl ammonium Exchanger

0.11meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X500

Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride)

1.6meq/gm

Superficially porous

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-2.0N

5,000 psi

PRP-X600

Poly (di-methyl amido-propyl meth-acrylamide)

1.6meq/gm

Superficially porous

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

RCX-10

PSDVB* with Tri-methyl ammonium Exchanger

0.35meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

RCX-30

PSDVB* with Tri-methyl ammonium Exchanger

1.0meq/gm

100 Å

pH Dependent**

pH 1-13. 0-100% aqueous, organic modifier

0.0-1.0N

5,000 psi

Cation Exchange Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X200

PSDVB* Sulfonate Exchanger

35ueq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

PRP-X400

PSDVB* Sulfonate Exchanger

2.5meq/gm

N/A

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-5.0N

5,000 psi

PRP-X800

PSDVB* Itaconate Exchanger

1.6meq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

HC-40 Calcium

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water

Water

1,000 psi

HC-75 Calcium

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

HC-75 Hydrogen

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

HC-75 Lead

PSDVB* Sulfonate Exchanger

5meq/gm

Gel-type

25-90 °C

100% Water 0-40% Acetonitrile

Water

400 psi

Ion Exclusion Columns

Packing Name

Support Material

Exchange Capacity

Pore Size

Temp. Limits

Phase Limits

Buffer Strength

Maximum Pressure

PRP-X300

PSDVB* Sulfonate Exchanger

0.17meq/gm

100 Å

5-60 °C

pH 1-13. 0-100% aqueous, organic modifier

0.0-0.5N

5,000 psi

*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C

 

 

 

HPLC色譜柱保養(yǎng)和儲存

 

HPLC柱具有有限的壽命。建議您使用標準定期監(jiān)測色譜柱的保留特性和性能。如果您打算長時間儲存??色譜柱,請使用抑制微生物生長的流動相(含有sodium azide或高濃度甲醇,乙腈等的流動相)。為獲得性能和色譜柱壽命,請遵循以下建議。

  • 定期監(jiān)控色譜柱的性能。
  • 僅在可混溶的流動相之間切換。
  • 避免在色譜柱中沉淀鹽。
  • 使用過濾和脫氣的流動相。
  • 不要讓色譜柱變干。不使用時請將其蓋緊。
  • 對于長期儲存,使用抑制細菌和霉菌生長的流動相。
  • 按標簽上指示的方向使用該列。異常高的操作壓力可能表明插入的入口玻璃料堵塞,并且可以通過使流過柱的流量反轉10-20mL來清除。
  • 使用Hamilton保護柱去除可能終身性結合分析柱的顆粒物質或雜質。

恢復色譜柱性能
下面列出的步驟可能會恢復已經污染的色譜柱的性能,但這些過程不會改善損壞或超出其使用壽命的色譜柱的性能。也不能恢復所有固定相。PRP-X500和PRP-X600色譜柱的*支持使得無法通過洗滌程序恢復性能。有關這些色譜柱的技術幫助,請HPLC支持。注意:在執(zhí)行恢復程序之前,請務必確保流動相是可混溶的,并且不會發(fā)生沉淀。如有必要,在轉換到新的流動相之前,先用合適的中間溶劑清洗色譜柱。應使用至少五個柱體積的中間溶劑。

色譜柱修復程序
按照下面列出的適當修復程序,使用1.0或2.0 mL / min的流速。除非另有規(guī)定。使用每列隨附的性能測試報告中列出的測試混合物和條件評估色譜柱性能。

柱修復程序

Packing Material Name

 

Restoration Procedure

PRP-C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-1

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-3

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-h5

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

HxSil C8

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

HxSil C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes.

PRP-X100

Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X200

Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X300

Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes.

PRP-X400

Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes.

RCX-10

Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.

RCX-30

Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes.

HC-40

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

HC-75

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

H+      Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

Pb2+  Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes.

 

 

 

 

PRP-X100色譜柱

 

零件號/ REF 

描述 

79641

分析型HPLC色譜柱,CUSTOM ORDER(1.0,2.1,4.1,4.6 mm ID)

79669

負離子快

79670

負離子微

79668

負離子分辨率

79643

制備型HPLC色譜柱#1,定制(21.2,30 mm內徑)

79644

制備型HPLC色譜柱#2,定制(50 mm內徑)

79645

制備型HPLC色譜柱#3,CUSTOM ORDER(100 mm ID)

79421

PRP-X10010μm2.1x 150 mm

79346

PRP-X10010μm2.1x 250 mm

79439

PRP-X10010μm4.1x 100 mm

79434

PRP-X10010μm4.1x 150 mm

79433

PRP-X10010μm4.1x 250 mm

79365

PRP-X10010μm4.1x 50 mm

79354

PRP-X10010μm4.6x 150 mm PEEK

79455

PRP-X10010μm4.6x 250 mm PEEK

79353

PRP-X10012-20μm100Å21.2x 250 mm

79852

PRP-X1005μm2.1x 150 mm PEEK

79190

PRP-X1005μm2.1x 250 mm

79538

PRP-X1005μm4.1x 100 mm

79812

PRP-X1005μm4.1x 150 mm

79810

PRP-X1005μm4.1x 50 mm

79174

PRP-X1005μm4.6x 150 mm PEEK

79181

PRP-X1005μm4.6x 250 mm PEEK

79665

PRP-X1007μm4.6x 150 mm PEEK

79642

半制備型HPLC色譜柱,CUSTOM ORDER(7.0,7.8,10 mm ID)

 

 

貨號

品名

79477

Analytical Guard Cartridge Holder, PEEK

32908

Analytical Guard Cartridge Holder, Stainless Steel

79293

Analytical Guard Column, PRP-X110

79641

Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID)

79643

Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID)

79644

Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID)

79645

Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID)

79732

PRP-X110 7 µm 4.1 x 150 mm

79734

PRP-X110 7 µm 4.1 x 250 mm

79738

PRP-X110 7 µm 4.6 x 150 mm PEEK

79729

PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK

79728

PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel

79727

PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK

79726

PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel

79827

PRP-X110, 7 µm Bulk Resin

79743

PRP-X110S 7 µm 2.1 x 100 mm PEEK

79733

PRP-X110S 7 µm 4.1 x 150 mm

79735

PRP-X110S 7 µm 4.1 x 250 mm

79741

PRP-X110S 7 µm 4.6 x 250 mm PEEK

79642

Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID)

 

 

 

 

 

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